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KMID : 0617219990100010039
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Duksung Bulletin Phamaceutical Sciences
1999 Volume.10 No. 1 p.39 ~ p.46
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Curcumin Inhibits Invasive Phenotype of H-ras Transformed Human Breast Epithelial Cells and Suppresses Matrix Metalloproteinase-2 Activity
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Kim Mi-Sung
Aree Moon
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Abstract
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A growing number of recent studies have focused on anticarcinogenic, antimutagenic or chemopreventive activities of phytochemicals, particularly those included in human diet. Curcumin, a dietary pigment in turmeric, has been shown to have a wide range of biological activities including anti-inflammatory, anticarcinogenic and antimetastatic effects. In the present study, we attempted to study the possible anti-invasive activity of curcumin in H-ras transformed MCF10A human breast epithelial cells which have previously been shown to be highly invasive. Here, we show that curcumin inhibits H-ras-induced invasive phenotype in MCF10A cells. A significant downregulation of matrix metalloproteinase (MMP)-2 by curcumin is observed while the activity of MMP-9 is not changed, suggesting that MMP-2 is more likely involved in the inhibitory effect of curcumin than MMP-9. We also show that curcumin treatment inhibits cell growth dose-dependently and induces internucleosomal DNA fragmentation in H-ras MCF10A cells and MCF7 human breast carcinoma cells. These results demonstrate that curcumin induces apoptosis in transformed human breast epithelial cells and breast carcinoma cells, suggesting a potential use of curcumin as a chemopreventive agent for breast cancer.
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